Wayne S. Hill wrote:

Mike V wrote:


At first glance, I would have taken the "February 2004
edition of the Journal of Applied Physiology" as moderately
current. Just shows how much I know. Getting dated myself, I
suppose. MikeV


Just because the journal has a current date doesn't mean that
its contents are anything new.

I was optimistic in saying 1995. Try 1987.

Lyle

***
J Clin Invest. 1987 Apr;79(4):1062-9.

Differential effects of hyperinsulinemia and hyperaminoacidemia on
leucine-carbon metabolism in vivo. Evidence for distinct mechanisms in
regulation of net amino acid deposition.

Tessari P, Inchiostro S, Biolo G, Trevisan R, Fantin G, Marescotti MC,
Iori E,
Tiengo A, Crepaldi G.

The effects of physiologic hyperinsulinemia and hyperaminoacidemia,
alone or in
combination, on leucine kinetics in vivo were studied in postabsorptive
healthy
subjects with primed-constant infusions of L-[4,5-3H]leucine and
[1-14C]alpha-ketoisocaproate (KIC) under euglycemic conditions.
Hyperinsulinemia
(approximately 100 microU/ml) decreased (P less than 0.05 vs. baseline)
steady
state Leucine + KIC rates of appearance (Ra) from proteolysis, KIC
(approximately leucine-carbon) oxidation, and nonoxidized leucine-carbon
flux
(leucine----protein). Hyperaminoacidemia (plasma leucine, 210
mumol/liter), with
either basal hormone replacement or combined to hyperinsulinemia,
resulted in
comparable increases in leucine + KIC Ra, KIC oxidation, and
leucine----protein
(P less than 0.05 vs. baseline). However, endogenous leucine + KIC Ra was
suppressed only with the combined infusion. Therefore, on the basis of
leucine
kinetic data, hyperinsulinemia and hyperaminoacidemia stimulated net protein
anabolism in vivo by different mechanisms. Hyperinsulinemia decreased
proteolysis but did not stimulate leucine----protein. Hyperaminoacidemia
per se
stimulated leucine----protein but did not suppress endogenous
proteolysis. When
combined, they had a cumulative effect on net leucine deposition into body
protein.

PMID: 3549777 [PubMed - indexed for MEDLINE]